Chitinolytic Activities in Trichomonas Vaginalis
نویسنده
چکیده
Chitinolytic activities were identified in the Protozoa Trichomonas vaginalis. Overall chitinase activity assessed using chitine-azure as substrate was 10.93 ± 1.21 nmoles/min/mg prot. End nonreducing chitobiosidase (exochitinase) and chitotriosidase (endochitinase) activities were shown using p-nitrophenyl-substrates and had specific activities of 4 .55 ± 0.53 and 0.47 ± 0.06 nmol/min/mg prot, Kmapp.= 1.32 mM and 5 μM and pH optimum = 7.0 and 6.1 respectively. ß-N-acetylhexosaminidase (NAHase) activity was also detected with a specific activity of 5.40 ± 0.65 nmol/min/mg prot., Kmapp = 0 .656 mM and pH optimum at 7.0. No release ol these enzymes into the culture medium was found. The possible role of chitinases in T. vaginalis remains to be investigated.
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